NGAL ELISA Kit (human)

Cat.No. KIT 036

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Cat. No.
KIT 036
Product name
NGAL ELISA Kit (human)
Description
A quantitative assay for the in vitro determination of human NGAL in urine, serum, plasma, tissue extracts or culture media.
Assay range
10-1000 pg/mL
Assay time
<4 hours
Limit of detection
4 pg/mL
Sample dilution
1/500 (Urine from healthy subjects)
Species reactivity (positive)
None
Storage
4°C
Target
Human NGAL (Neutrophil Gelatinase-Associated Lipocalin)
Background
NGAL is a potential new biomarker for the acute kidney injury (AKI).
 
Under normal conditions, NGAL levels are low in urine and plasma, but they rise sharply from basal levels in response to kidney injury and peaks within a very short time – as much as 24 hours or more before any significant rise in serum creatinine. The finding of a raised urinary or plasma level of NGAL cannot be independently diagnostic of any single pathology, since a variety of independent pathologies (e.g. inflammation, infection and certain types of cancer) are associated with raised levels of urinary or plasma NGAL.
Regulatory status
RUO - Research Use Only. Not for use in diagnostic procedures
Other information
Kit features Simple ELISA format - easy to perform Ready-to-use calibrators and working solutions Pre-coated ELISA strips All incubations performed at room temperature Easy storage All components are stored at 4°C Automation possible on open ELISA workstations
Cho E, Yang HN, Jo SK, Cho WY, Kim HK, (2013) The role of urinary liver-type fatty acid-binding protein in critically ill patients. J Korean Med Sci 
Di NM, Ficarella A, Ricci Z, Luciano R, Stoppa F, Picardo S, Picca S, Muraca M, Cogo P (2013) Impact of severe sepsis on serum and urinary biomarkers of acute kidney injury in critically ill children: an observational study1. Blood Purif 
Kashani K, Al-Khafaji A, Ardiles T, Artigas A, Bagshaw SM, Bell M, Bihorac A, Birkhahn R, Cely CM, Chawla LS, Davison DL, Feldkamp T, Forni LG, Gong MN (2013) Discovery and validation of cell cycle arrest biomarkers in human acute kidney injury.Crit Care
Kift RL, Messenger MP, Wind TC, Hepburn S, Wilson M, Thompson D, Smith MW, Sturgeon C, Lewington AJ, Selby PJ, Banks RE (2013) A comparison of the analytical performance of five commercially available assays for neutrophil gelatinase-associated lipocalin using urine. Ann Clin Biochem 
Hasse-Fielitz A, Haase M, Devarajan P (2014) Neutrophil gelatinase-associated lipocalin as a biomarker of acute kidney injury: a critical evaluation of current status. Ann Clin Biochem [Epub ahead of print]